Dr Kieran J Germaine

PhD, BSC

 
Assistant Lecturer in Biosciences

e: kieran.germaine@itcarlow.ie t: 059 915580

 
  • Research Interests
  • Publications
  • Research Supervision

Industrial Research Experience

  • 2009-2012           Post-doctoral Scientist, IT Carlow
  • 2008-2009           Post-doctoral Scientist, Dublin Institute of Technology (DIT)
  • 2006-2008           Pharmaceutical Microbiologist, Schering-Plough, Rathdrum, Co. Wicklow
  • 2005-2006           Post-doctoral Scientist, IT Carlow
  • 2002-2005           Antibiotic and Hormone Residue Analyst, Foodtech laboratories Ltd. Blanchardstown, Dublin
  • 1999-2002           Food Microbiologist. Foodtech laboratories Ltd. Blanchardstown, Dublin, Ireland

My research interests includes a diversity of topics including isolating and characterising organic pollutant degrading bacteria, isolating heavy metal resistant bacteria, plant growth promoting  bacteria and their growth promoting traits,  bacterial genomics, uses of plant associated bacteria as biocontrol agents, bacterial biosensors, plant microbiomes, waste water bioremediation, human pathogens survival in the plant biosphere, control of human pathogens  in meat using essential oils and genetic characterisation of Brassica napus.

Publications

1. Oteino, N., Lally R, Kiwanuka S, Lloyd A, Ryan D, Germaine KJ and Dowling, DN. (2015).   Phenotypic, genotypic and In Planta analysis of the phosphate solubilisation ability of endophytic bacterial strains.  (In preparation).

2. Liu X,  Kiwanuka S., Ryan D., Dowling D N. and  Germaine K. (2015). Use of ecopiling in a field trial to remediate heavily impacted creosote contaminated sediment and soil. (In preparation).

3. Germaine KJ, Liu X, Byrne J, Culhane J, Lally R, Kiwanuka S, Keohane J, Ryan D, and Dowling, DN. (2014) Ecopiling: A combined phytoremediation and passive biopiling system for remediating hydrocarbon impacted soil at field scale. Frontiers in Plant Science. 5:756-762.

4. Lally R. Moreira AS, Galbally P, Ryan D, Germaine KJ and Dowling, DN. (2013). Application of Endophytic Bacteria Possessing Plant Growth Promoting Traits for the Use in Bioenergy Crop Brassica napus. Aspects of Applied Biology 120, 9-14.

5. Oteino N, Culhane, J., Germaine, KJ, Ryan, D, Brazil, D and Dowling, DN. (2013).Screening of large collections of plant associated bacteria for effective plant growth promotion and colonisation. Aspects of Applied Biology 120, 23-28.

6. Redondo-Nieto M, Barret M, Morrissey J, Germaine K, Martínez-Granero F, Barahona E, Navazo A, Sánchez-Contreras M, Moynihan JA, Muriel C, Dowling D, O'Gara F, Martín M, Rivilla R. (2013) Genome sequence reveals that Pseudomonas fluorescens F113 possesses a large and diverse array of systems for rhizosphere function and host interaction.  BMC Genomics. 25:14:54. doi: 10.1186/1471-2164-14-54.

7. El-Sawi, Germaine KJ, Bourke P and Malone R.A (2012) Assessment of Morphological Variation in Irish Brassica oleracea species. Journal of Agricultural Science 4:10. DOI: 10.5539/jas.v4n10p20

8. Power B, Liu X, Germaine KJ, Ryan D, Brazil D and Dowling DN (2011). Alginate beads as a storage, delivery and containment system for genetically modified PCB degrader and PCB biosensor derivatives of Pseudomonas fluorescens F113. J. Appl. Microbiol. 110 1351-1358.

9. Liu X, Germaine K, Ryan D and Dowling D N (2010). Whole-Cell fluorescent biosensors for bioavailability and biodegradation of polychlorinated biphenyls. Sensors 2010, 10, 1377-1398; doi:10.3390/s100201377.

10. Liu X, Germaine KJ, Ryan D and Dowling D (2010). Genetically modified Pseudomonas biosensing biodegraders to detect PCB and chlorobenzoate bioavailability and biodegradation in contaminated soils. Bioengineered Bugs 1:3, 1-9.

11. Germaine KJ, Keogh E, Ryan D and Dowling D N (2009). Bacterial endophyte-mediated naphthalene phytoprotection and phytoremediation. FEMS Microbiol. Lett. 2009, 296, 226-234.

12. Germaine, K., Ryan, D., Dowling, D. N. (2008). Endophyte mediated naphthalene phyto-protection and phyto-remediation. FEMS Microbiol. Ecology.

13. Liu, X., Germaine, K., Ryan, D., Dowling, D. N. (2007). Development of an alginate encapsulated gfp-based biosensor for detecting the bioavailability and biodegradation of polychlorinated biphenyls. Journal of Environmental Engineering and Landscape Management. Vol XV, No 4, 261–268.

14. Germaine, K., Liu, X., Cabellos, G., Ryan, D., Dowling, D. N. (2006). Bacterial endophyte enhanced phytoremediation of the organochlorine herbicide 2, 4 dichlorophenoxyacetic acid. FEMS Microbiol. Ecology. 57:302-310.

15. Garcia–Cabellos, G., Germaine, K., Ryan, D., Dowling, D. N. (2005). Investigating the rhizosphere microbiology of constructed wetlands. Symposium proceedings publication: Nutrient Management in Agricultural Watersheds.

16. Germaine, K., Keogh, E., Cabellos, G., Borremans, B., van der Lelie, D., Barac, T., Oeyen, L., Vangronsveld, J., Porteous Moore, F., Moore, E., Campbell, C., Ryan, D., and Dowling, D.N. (2004). Colonisation of poplar trees by gfp expressing bacterial endophytes. FEMS Microbiology Ecology 48: 109–118.

Review Papers

1. Germaine KJ, Chhabra S, Song B, Brazil D and Dowling D N (2010). Microbes and sustainable production of biofuel crops: a Nitrogen perspective. Biofuels. 1: 6: 877-888.

2. Ryan, R., Germaine, K., Franks, A., Ryan, D., Dowling, D. N. (2008). Bacterial endophytes: recent developments and applications. FEMS Microbiology Letters. 278: 1-9.

Book Chapters

1. Germaine, KJ, McGuinness, M, and Dowling, DN. (2013). Improving phytoremediation through plant-associated bacteria. In: Molecular Microbial Ecology of the rhizosphere. Wiley-Blackwell. New Jersey. Chpt. 91. ISBN: 978-1-118-29629-5.

2. Germaine, KJ, McGuinness, M, and Dowling, DN. (2013). Engineering of microbes for plant and soil systems. In: Applications of microbial engineering (Eds: VK Gupta, M Schmoll, M Maki, M Tunhy and MA Mazutti). Chpt 8: CRC Press.

3. Germaine KJ and Dowling D N (2010). Improving the phytoremediation potential of plants using endophytic bacteria in Trends in Bioremediation and Phytoremediation, (Editors: Grażyna Płaza). 2010: ISBN: 978-81-308-0424-8.

Credits

Gfp microraphs used in: Estibaliz Larrainzar, E., ­ O'Gara, F. and ­ Morrissey, J. (2005). Applications of autofluorescent proteins for in situ studies in microbial ecology. Annual Review of Microbiology. 59: 257-277.

Gfp micrograph featured on front cover of Nature Biotechnology. May, 2004, Volume 22 No 5.

Patents

Ecopiling: A combined phytoremediation and passive biopiling system for remediating hydrocarbon impacted soil. Patent Application No.: S2140311  (patent pending).

Current Projects

1. Investigation of the Microbiome associated with Oilseed rape crops. Ms. Ridhdhi Rathore. PhD candidate.  Walsh Funding Teagasc. Sept 2013-Sept 2016.

2. Genome analysis and data mining of two endophytic bacteria. Mr. Samuel Kiwanuka. PhD candidate.  IOTI Postgraduate Fellowship Award. Sept 2013-Sept 2016.

3. Bioinformatic analysis of plant growth promotion (PGP) associated sequences in a commercially significant PGP microorganism and the development of a unique biomarker for in-situ monitoring. Mr.  Richard Lally. PhD candidate.  IOTI/ITC Postgraduate Fellowship Award. Sept 2013-Sept 2016

4. Feasibility study to test the efficiency, reliability and commercial potential of Microbial Enhanced BioSwale (MEBS) systems for the treatment of Farmyard Dirty Water. Research Assistants: Ms. Roseanna Merriman (MSc) and Carme Arnabat (MSc). Science foundation Ireland (SFI) Technology Innovation and Development Award. Jan 2013-May 2015. 

5. Feasibility study to test the commercial potential of microbial biofertilisers in Irish crops under field trial conditions. Post-doctoral researchers: Dr. Antonio Sergio Moreira and Dr. Paul Galbally. Science foundation Ireland (SFI) Technology Innovation and Development Award. Jan 2011-May 2013. 

Industry collaborations/Projects to-date

1. Cu Chulainn Hurleys Ltd. Inch Mills, Sion Road, Kilkenny (current): This is an innovation voucher project that has just started ad involves the production of fungi inoculants for treating beech wood in order to produce decorative wood patterns in timber, which triples its economic value.

2. Ocean leaves Ltd. Thornville Killinick, Co. Wexford (March-September 2014). This was an innovation voucher project investigating the potential of seaweed extracts as plant growth stimulants.

3. Microgen Biotech Ltd, IT Carlow (June 2014- present): This project involved the bioremediation of crude oil contaminated sludge in Shandong Province China.

4. CeresH2O (Florida, USA) and BlueBarrel Ltd., Dublin Ireland.(2012-Present); This project involves the design , construction and monitoring of a novel bioswale system for treating wastewater.

5. Verde Environmental Group (June 2012-October 2012). This project involved a pilot scale remediation trial of creosote contaminated sediment from a former wood treatment facility in Ireland.

6. Inter Euro Technologies Ltd (November 2012-April 2013): GC detection of novel synthetic marker compounds in green diesel.

7. Eddie O’Gorman Ltd, Kilkenny (December 2011-April 2012): This innovation voucher project involved the analysis of colloidal creatine products in solid gels.

8. Eddie O’Gorman Ltd, Kilkenny (December 2010-April 2011): This innovation voucher project involved the analysis of colloidal creatine products in liquid gels.

9. Independent Seed Company (Kildare) and Feldsaaten Freudenberger Gmb (Germany) (2011-2012): Development of coated Oilseed rape containing live microbial biofertiliser strains.

10. GES Ltd, Carlow (2009-2013): This large scale project involved the bioremediation of oil contaminated soil at the former Irish sugar site in Carlow.

Membership of Professional Bodies