Dr Patricia Mulcahy

B.Sc., Ph.D., C. Biol., FIFSTI

President of IT Carlow
 
 
 
  • Research Interests
  • Publications
  • Research Supervision

Research Interests

Dr Patricia Mulcahy established the Biocatalyst Technology Group at the Institute of Technology Carlow in 1992 as part of the Institutes Research Programme in Biotechnological and Molecular Environmental Sciences.
 
Her research interests and activities are focused primarily on the design of high affinity kinetic-based systems for the analyses, purification and study of cofactor-dependent iocatalysts and development of the biotechnological potential of selected enzymes.
 
This includes research into the design, synthesis and characterisation of high affinity and specific immobilised ligands for chromatographic and protein chip technologies (class-specific protein microarrays), identification and biochemical characterisation of novel microbial NAD(P)+-dependent biocatalysts, and development of enzyme technological applications.
 
The group is also active in the area of biofuel production research where it has been developing biological processes for the conversion of lignocellulosic materials to ethanol for use as a fuel / fuel additive in collaboration with industry. Fundamental aspects of microbial physiology and fermentation technology are being integrated with molecular biochemistry and enzymology, to further understand the key elements of microbial pentose metabolism and to work towards the development of new microbial strains with improved properties for the rapid and efficient conversion of pentose sugars to ethanol.

 

Research Publications

 
Forde, J., Oakey, L. and Mulcahy, P. (2005)
Fundamental differences in bioaffinity of amino acid dehydrogenases for N6 and S6 -linked immobilised cofactors using KBECS. Analytical Biochemistry, 338, 102-112
 
Oakey, L. and Mulcahy, P. (2004)
Immobilised cofactor derivatives for kinetic-based enzyme capture strategies: Direct coupling of NAD(P)+. Analytical Biochemistry, 335, 316-325
 
McMahon, M., Tynan, J. and Mulcahy, P. (2003)
Kinetic locking-on and auxiliary tactics for bioaffinity purification of NADP+- dependent dehydrogenases using N6-linked immobilised NADP+-derivatives: Studies with mammalian and microbial glutamate dehydrogenases. Biotechnology and Bioengineering, 81, 3, 356-369.
 
Mulcahy, P., OFlaherty, M., Jennings, L. and Griffin, T. (2002)
Application of kinetic-based biospecific affinity chromatographic systems to ATP-dependent enzymes, Analytical Biochemistry, 309, 2, 279-292.
 
McMahon, M. and Mulcahy, P. (2002)
Bioaffinity purification of an NADP+-dependent secondary alcohol dehydrogenase from Thermoanaerobacter brockii using kinetic locking-on and auxiliary tactics: A comparative study of N6, N1, 8azo and C8-linked immobilised cofactor derivatives. Biotechnology and Bioengineering, 77, 517-527.
 
Mulcahy, P. and OFlaherty, M. (2001)
Prospective: The kinetic locking-on strategy and auxiliary tactics for bioaffinity purification of NAD(P)+-dependent dehydrogenases. Invited review for Analytical Biochemistry, 299, 1-18.
 
Tynan, J., Forde, J., McMahon, M. and Mulcahy, P.
2000Synthesis of a highly substituted N6-linked immobilised NAD+ derivative using a rapid solid-phase modular approach: Suitability for use with the kinetic locking-on tactic for bioaffinity purification of NAD+-dependent dehydrogenases. Protein Expression and Purification, 20, 421-434.
 
Mulcahy, P., OFlaherty, M., McMahon, M. and Oakey, L. (1999)
Effect of accessible immobilised NAD+ concentration on the 
bioaffinity chromatographic behavior of NAD+-dependent dehydrogenases using the kinetic locking-on strategy. Protein Expression and Purification, 16, 261-275.
 
Oakey, L., Martung, A., McMahon, M., OFlaherty, M. and Mulcahy, P. (1999)
A comparison of biospecific affinity chromatographic methodologies for the purification of NAD+-dependent dehydrogenases; Studies with bovine L-lactate dehydrogenase. Process Biochemistry, 35, 349-357.
 
OFlaherty, M., McMahon, M., Forde, J. and Mulcahy, P. (1999)
The kinetic locking-on strategy for bioaffinity purification; Further studies with bovine liver glutamate dehydrogenase. Protein Expression and Purification, 16, 276-297.
 
OFlaherty, M., McMahon, M and Mulcahy, P. (1999)
A kinetic locking-on strategy for bioaffinity purification; Further 
studies with alcohol dehydrogenase. Protein Expression and 
Purification, 15, 127-145.
 
OFlaherty, M., OCarra, P., McMahon, M and Mulcahy, P. (1999)
A stripping ligand tactic for use with the kinetic locking-on 
strategy: Its use in the resolution and purification of 
NAD+-dependent dehydrogenases. Protein Expression and Purification, 16, 424-431.
 
Mulcahy, P. and OCarra, P. (1997
Purification and substrate kinetics of plant lactate dehydrogenase. Phytochemistry, 45, 5, 889-896.
 
Mulcahy, P., Cullina, A. and OCarra, P. (1997)
Both D- and L-lactate specific dehydrogenases coexist in individual cephalopods. Comparative Biochemistry and Physiology. [A], 116, 2, 143 - 148.
 
Mulcahy, P., Griffin, T. and OCarra, P. (1997)
Biospecific affinity purification of octopine dehydrogenase from 
molluscs. Protein Expression and Purification. 9, 1, 109-114.
 
OCarra, P. and Mulcahy, P. (1997)
Plant lactate dehydrogenase: Kinetics with NADH and inhibition by ATP. Phytochemistry, 45, 5, 897-902.
 
OCarra, P. and Mulcahy, P. (1996)
Plant lactate dehydrogenases: Distribution and Function. 
Phytochemistry, 42, 581-587.
 
OCarra, P., Griffin, T., OFlaherty, M., Kelly, N. and Mulcahy, 
P. (1996)
Further studies on the biospecific affinity chromatographic purification of NAD+-dependent dehydrogenases using the locking-on tactic. Biochimica et Biophysica Acta, 1297, 235-243.